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Image Search Results
Journal: Molecular Biomedicine
Article Title: Activated oligoadenylate synthetase-ribonuclease L pathway promotes endothelial pyroptosis and impairs diabetic wound healing via thioredoxin-interacting protein m 6 A methylation
doi: 10.1186/s43556-025-00399-9
Figure Lengend Snippet: Elevated expression of OSA-RNase L in the HUVECs stimulated by high glucose. Immunofluorescence assay and semi-quantitative analysis of dsRNA ( a - b ). dot blot assay of dsRNA in the diabetic mouse skin ( c ). RT-qPCR assay of OAS1 mRNA ( d ), OAS2 mRNA ( e ), OAS3 mRNA ( f ), RNase L mRNA ( g ) expression in HUVECs stimulated by high glucose. Western blot assay of OAS1, OAS2, OAS3 and RNase L protein ( h ), and immunofluorescence assay and semi-quantitative analysis of OAS1, OAS2, OAS3 and RNase L (i-p). The results are presented as the mean ± SEM, * p < 0.05, ** p < 0.01, **** p < 0.0001. Each experiment was replicated for thrice. The number of mice used in this experiment was four per group
Article Snippet: The membranes were incubated overnight at 4 °C with specific primary antibodies: OAS1 (14955-1-AP),
Techniques: Expressing, Immunofluorescence, Dot Blot, Quantitative RT-PCR, Western Blot